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KMID : 0615720120290010127
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Jouranl of Korean Acupuncture and Moxibustion society
2012 Volume.29 No. 1 p.127 ~ p.137
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The Effect of Clematidis Radix Herbal-acupuncture Solution, on Collagen, Adjuvant, Lipopolysaccharide and Phospholipase A2 Induced Rheumatoid Arthritis in Mice
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Lee Jin-Seok
Kim Kyung-Ho
Lee Seung-Deok
Kim Kap-Sung
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Abstract
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Objectives: The purpose of this study is to investigate the effect of Clematidis radix herbal-acupuncture solution, on collagen, adjuvant, lipopolysaccharide and phospholipase A2 induced rheumatoid arthritis in mice.
Methods:Arthritis index was measured for mouse that was injected subcutaneously in solution mixed chicken type Ⅱ collagen with Freund’s complete adjuvant. We injected Freund’s complete adjuvant into right posterior part of the sole of a ICR mouse foot, which was measured by plethysmometer. The solution mixed CRHS with Tris-HCI, CaCl2, substrate, enzyme was done a chemical action for thirty minutes, and then inhibitory activity of PLA2 enzyme was expressed with inhibition percentage by utilizing isolated arachidonic acid. COX-2 was induced by adding LPS to RAW 264.7 cell, and COX-2 activity was measured by western blot analysis and PGE2 Biotrak kit.
Results: CRHS also inhibited Freund’s complete adjuvant induced chronic rheumatoid arthritis in mice. CRHS showed significant inhibition of type Ⅰ and type Ⅱ PLA2 activities in a dose dependent manner. Furthermore, PGE2 production was decreased with CRHS and lipopolysaccharide-induced COX-2 protein expression was significantly inhibited by CRHS.
Conslusions: These results suggest that CRHS has an therapeutic effect on drug induced-rheumatoic arthritis by inhibiting PLA2 and COX-2 activities.
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KEYWORD
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rheumatoid arthritis, Clematidis radix herbal-acupuncture solution, CRHS, phospholipase A2 activity, cyclooxygenase-2
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